Stress Biomarkers in Soccer - Part 2: Dosage of Oxidative Stress Blood Levels
Abstract
The dissemination of scientific methods has always been a great dilemmas in applied research. The information tend to be brief, since the consensus is that a sector of the scientific community share the same know-how. However, it is normal for early stage researchers in contact with methodological procedures as diverse area of Biochemistry of Exercise may have difficulties in understanding and implementation of specific analyzes. The objective of this paper is to present theoretical arguments about the importance of oxidative stress in the onset phase of overreaching (metabolic stress) in soccer, as well as detail the steps for analysis of important biomarkers of stress in this category.
References
-Aebi, H. Catalase in vitro. Methods in Enzymology. Vol. 105. p. 121-126. 1984.
-Alessio, H. M. Exercise-induced oxidative stress. Medicine and Science in Sports and Exercise. Vol. 25. p. 218-224. 1993.
-Antunes Neto, J. M. F. Modificações morfo-funcionais do tecido muscular induzidas pela atividade excêntrica: um estudo global dos processos adaptativos. Dissertação de Mestrado. Faculdade de Educação Física da Universidade Estadual de Campinas. 1998.
-Antunes Neto, J. M. F. Estudo da relação entre estresse oxidativo e síntese de proteínas de estresse “HSP70” em animais submetidos a diferentes níveis de exercício físico. Tese de Doutorado. UNICAMP. Instituto de Biologia. Departamento de Bioquímica. 2003.
-Antunes Neto, J. M. F.; Nader, B. B.; Donadon, C. C.; Macedo, D. V. Biomarcadores de estresse no futebol: dosagem sangüínea dos níveis de creatina quinase. Vol. 4. Num. 12. p. 87-97. 2012.
-Antunes Neto, J. M. F.; Pereira-da-Silva, L.; Macedo, D. V. Biomarcadores de estresse oxidativo: novas possibilidades de monitoramento em treinamento físico. Revista Brasileira de Ciência e Movimento. Vol. 13. Num. 2. 2005. p. 73-79.
-Antunes Neto, J. M. F.; Toyama, M. H.; Carneiro, E. M.; Boschero, A. C.; Pereira-da-Silva, L.; Macedo, D. V. Circulating leukocyte heat shock protein 70 (HSP70) and oxidative stress markers in rats after a bout of exhaustive exercise. Stress. Vol. 9. p. 107-115. 2006.
-Ästrand, P. O. Why exercise?Medicine and Science in Sports and Exercise. Vol. 24. p. 153-162. 1992.
-Atalay, M.; Marnila, P.; Lilius, E. M. Glutathione-dependent modulation of exhausting exercise-induced changes in neutrophil function in rats.European Journal of Applied Physiology. Vol. 74. p. 342-346. 1996.
-Beckman, J. S.; Beckman, T.; Freeman, B. Apparent hydroxyl radical production by peroxynitrite: implications for endotelial injury from nitric oxide and superoxide.Proc. Natl. Acad. Scie. USA. Vol. 87. p. 1620-1624. 1990.
-Beutler, E. Red cell metabolism. In: A Manual of Biochemical Methods. London: Grune & Stratton Publishers, 1975.
-Bruin, G.; Kuipers,H.; Keizer, H. A.; Vandervusse, G. J. Adaptation and overtraining in horses subjected to increasing training loads. Journal of Applied Physiology. Vol. 76. Num. 5. p. 1908-1913. 1994.
-Buczynsky, A.; Blaszczyk, J.; Kedziora, J. Blood platelet superoxide dismutase activity and malonyldialdehyde concentrations in healthy men following 112 submaximal physical exercise. In: Nazar, K.; Terjung, R. L.; Uscilko, H. K. e colaboradores.(orgs.). International perspectives in exercise physiology. Champaign: Human Kinetics, 1990.
-Criswell, D.; Powers, S.; Dodd, S.; Lawler, J.; Edwards, W.; Renshler, K.; Grinton, S. High intensity training-induced changes in skeletal muscle antioxidant enzyme activity.Medicine and Science in Sports and Exercise. Vol. 25. p. 1135-1140. 1993.
-Davies, K.J. A.; Quintanilha, A. T.; Brooks, G. A.; Packer, L. Free radicals and tissue damage produced by exercise. Biochemical and Biophysical Research Communications. Vol. 107. p. 1198-1205. 1982.
-Dean, R. T. A mechanism for accelerated degradation of intracellular proteins after limited damage by free radicals. FEBS Letter. Vol. 220. p. 278-282. 1987.
-Faure, P.; Lafond, J. L. Measurement of plasma sulfhydryl and carbonyl groups as a possible indicator of protein oxidation. In: Favier, A.E.; Cadet, J.; Kalyanaraman, B.; Fontecave, M.; Pierre, J. L. (eds.) Analysis of Free Radicals in Biological Systems.Basel:Birkhäuser Verlag. p. 237-248. 1995.
-Goldfarb, A. H. Antioxidants: role of supplementation to prevent exercise-induced oxidative stress. Medicine and Science in Sports and Exercise. Vol. 25. p. 232-236. 1993.
-Halliwell, B.; Gutteridge, J. M. Free radicals in biology and medicine. Oxford: Clarendon Press, 1989.
-Hellsten, Y.; Hansson, H. A.; Johnson, L. Increased expression of xanthine oxidade and insulin-like growth factor I (IGF-I) immunoreactivity in skeletal muscle after strenuous exercise in humans.Acta Physiologica Scandinavica. Vol. 157. p. 191-197. 1996.
-Jenkins, R. R.; Goldfarb, A. Introduction: oxidative stress, aging, and exercise.Medicine and Science in Sports and Exercise. Vol. 25. p. 210-212. 1993.
-Ji, L. L.; Mitchell, E. W. Glutathione and antioxidant enzymes in skeletal muscle: effects of fiber type and exercise intensity.Journal of Applied Physiology. Vol. 73. p. 1854-1858. 1992.
-Ji, L. L.; Stratman, F.; Lardy, H. Antioxidant enzymes system in rat liver and skeletal muscle: influence of selenium deficiency acute exercise and chronic training.Archives of Biochemystry and Biophysic. Vol. 263. p. 150-160. 1988.
-Laughlin, M. H.; Simpson, T.; Sexton, W. L.; Brown, O. R.; Smith, J. K.; Korthuis, R. Skeletal muscle oxidative capacity, antioxidant enzymes and exercise training.Journal of Applied Physiology. Vol. 68. p. 2337-2343. 1990.
-Lazarim, F. L.; Antunes Neto, J. M. F.; Silva, F. O. C.; Nunes, L. A. S.; Bassani-Cameron, A.; Cameron L, Alves, A. A., Brenzikofer, R.; Macedo, D. V. The upper values of plasma creatine kinase of professional soccer players during the Brazilian National Championship. Journal of Science and Medicine in Sport. Vol. 12. Num. 1. p. 85-90. 2009.
-Lehmann, M.; Foster, C.; Dickhuth, H. H.; Gastmann, U. Autonomic imbalance hypothesis and overtraining syndrome. Medicine and Science in Sports and Exercise. Vol. 30. Num. 7. p. 1140-1148. 1988.
-Ohkawa, H.; Nobuko, O.; Yagi, K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction.Analytical Biochemistry. Vol. 95. p. 351-358. 1979.
-Powers, S. K.; Criswell, D.; Lawlwe, J.; Ji, L. L.; Martin, D.; Herb, R. A.; Dudley, G. Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle.American Journal of Physiology. Vol. 266. p. R375-380. 1994.
-Powers, S. K.; Ji, L. L.; Leeuwenburgh, C. Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review.Medicine and Science inSports and Exercise. Vol. 31. p. 987-997. 1999.
-Radák, Z.; Tahara, S.; Nakamoto, H.; Ohno, H.; Sasvári, M.; Nyakas, C.; Goto, S. The effect of exercise training on oxidative damage of lipids, proteins and DNA in rat skeletal muscle: evidence for beneficial outcomes.Free Radical Biology & Medicine. v. 27, p. 69-74.1999.
-Rahman, T.; Hosen, I.; Towhidul, I.; Shekar, H. U. Oxidative stress and human health. Advances in Bioscience and Biotechnology. Vol. 3. p. 997-1019. 2012.
-Reid, M. B. Reactive oxygen and nitric oxide in skeletal muscle.News of Physiology and Science. Vol. 11. p. 114-119. 1996.
-Reid, M. B. Role of nitric oxide in skeletal muscle: synthesis, distribution and functional importance.Acta Physiologica Scandinavica. Vol. 162. p. 401-409. 1998.
-Reid, M. B. Redox modulation of skeletal muscle contraction: what we know and what we don’t.Journal of Applied Physiology. Vol. 90. p. 724-731. 2001.
-Reid, M. B.; Durham, W. J. Generation of reactive oxygen and nitrogen species in contracting skeletal muscle –potential impact on aging.Ann. N. Y. Acad. Sci. Vol. 959. p. 108-116. 2002.
-Sen, C. Oxidants and antioxidants in exercise.Journal of Applied Physiology. Vol. 79. p. 675-686. 1995.
-Sjödin, B.; Weling, H.; Apple, S. Biochemical mechanisms for oxygen free radical formation during exercise.Sports Medicine. Vol. 10. p. 236-254. 1990.
-Sjödin, B.; Westing, Y. H. Changes in plasma concentration of hypoxanthine and uric acid in man with short-distance running at various intensities.International Journal of Sports and Medicine. Vol. 11. p. 493-495. 1990.
-Smith, I. K.; Vierheller, T. L.; Thorne, C. A. Assay of glutathione reductase in crude tissue homogenates using 5,5’-dithiobis(2-nitrobenzoic acid). Analitical Biochemistry. Vol. 175. p. 408-413. 1988.
-Smolka, M. B.; Zoppi, C. C.; Alves, A. A.; Silveira, L. R.; Marangoni, S.; Pereira-da-Silva, L.; Novello, J. C.; Macedo, D. V. HSP72 as a complementary protection against oxidative stress induced by exercise in the soleus muscleof rats.American Journal of Physiology. Vol. 279. p. R1539-1545. 2000.
-Tiidus, P. M. Radical species in inflamation and overtraining.Canadian Journal of Physiology and Pharmacology. Vol. 76. p. 533-538. 1998.
-Yagi, K. A. A simple fluorimetric assay for lipoperoxide in blood plasma.Biochemistry and Medicine. Vol. 15. p. 212-216. 1976.
-Yu, B. P. Cellular defenses against damage from reactive oxygen species.Physiological Reviews. Vol. 74. p. 139-162. 1994.
-Zoppi, C. C.; Hohl, R.; Silva, F. C. C.; Lazarim, F. L.; Antunes Neto, J. M. F.; Stancanneli, M.; Macedo, D. V. Vitamin C and E supplementation effects in professional soccer players under regular training. Journal of the International Society of Sports Nutrition. Vol. 3. Num. 2. p. 37-44. 2006.
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